<asp:GridView ID="GridView1" runat="server" AutoGenerateColumns="false" AllowPaging="true" OnRowDataBound="Grid_RowDataBound"
>
<Columns>
<asp:BoundField DataField="Usuario" HeaderText="" />
<asp:BoundField DataField="Estado" HeaderText="" />
<asp:TemplateField>
<ItemStyle HorizontalAlign="Center" width="1%"/>
</asp:TemplateField>
</Columns>
</asp:GridView>
Protected Sub Grid_RowDataBound(ByVal sender As Object, ByVal e As System.Web.UI.WebControls.GridViewRowEventArgs)
If e.Row.RowType = DataControlRowType.DataRow Then
'por cada fila va insertando el hiperlink aqui controla si deseas que se visualize el hiperlink o no
Dim hlnk As HyperLink = New HyperLink
hlnk.NavigateUrl = ""
hlnk.ImageUrl = "IMG/tu imagen.jpg"
hlnk.ToolTip = "Tu tooltip"
e.Row.Cells.Item(3).Controls.Add(hlnk)
End If
End Sub